Custom Mentholation of Commercial Cigarettes for Research Purposes.

TitleCustom Mentholation of Commercial Cigarettes for Research Purposes.
Publication TypeJournal Article
Year of Publication2014
AuthorsMacGregor IC, Stanfill SB, Gordon SM, Turner DJ, Butler JM, Hanft EA, Kim H, Kroeger RR, Brinkman MC, Tefft ME, Clark PI, Buehler SS
JournalToxicol Rep
Volume1
Pagination1068-1075
Date Published2014 Jan 1
ISSN2214-7500
Abstract

In the U.S. menthol remains the sole permitted characterizing cigarette flavor additive in part because efforts to link menthol cigarette use to increased tobacco-related disease risk have been inconclusive. To perform definitive studies, cigarettes that differ only in menthol content are required, yet these are not commercially available. We prepared research cigarettes differing only in menthol content by deposition of L-menthol vapor directly onto commercial nonmenthol cigarettes, and developed a method to measure a cigarette's menthol and nicotine content. With our custom-mentholation technique we achieved the desired moderately high menthol content (as compared to commercial brands) of 6.7 ± 1.0 mg/g (n = 25) without perturbing the cigarettes' nicotine content (17.7 ± 0.7 mg/g [n = 25]). We also characterized other pertinent attributes of our custom-mentholated cigarettes, including percent transmission of menthol and nicotine to mainstream smoke and the rate of loss of menthol over time during storage at room temperature. We are currently using this simple mentholation technique to investigate the differences in human exposure to selected chemicals in cigarette smoke due only to the presence of the added menthol. Our cigarettes will also aid in the elucidation of the effects of menthol on the toxicity of tobacco smoke.

DOI10.1016/j.toxrep.2014.10.009
Alternate JournalToxicol Rep
PubMed ID25621204
PubMed Central IDPMC4303566
Grant ListR01 CA162085 / CA / NCI NIH HHS / United States